Flow cytometry - Revision history

Mikael Häggström: +Viability

2023-10-18T00:48:16Z

+Viability

← Older revision		Revision as of 20:48, 17 October 2023
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			==Viability==
			[[File:Flow cytometric viability by 7-AAD.png\|thumb\|Flow cytometry using 7-Aminoactinomycin D (7-AAD), wherein a lower signal indicates viable cells. Therefore, this case shows good viability.]]
			Viability of the cells in flow cytometry should be around 95% but not less than 90%.<ref>{{cite web\|url=https://medicine.yale.edu/immuno/flowcore/protocols/analysis/\|title=Flow cytometry (FACS) staining protocol (Cell surface staining)\|website=Yale School of Medicine - Yale Flow Cytometry\|accessdate=2023-10-17}}</ref>

	==CD4/CD8 ratio==		==CD4/CD8 ratio==
	A ratio of 1.5 and 2.5 is generally considered normal.<ref name="pmid29095912">{{cite journal\| author=McBride JA, Striker R\| title=Imbalance in the game of T cells: What can the CD4/CD8 T-cell ratio tell us about HIV and health? \| journal=PLoS Pathog \| year= 2017 \| volume= 13 \| issue= 11 \| pages= e1006624 \| pmid=29095912 \| doi=10.1371/journal.ppat.1006624 \| pmc=5667733 \| url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=29095912 }} </ref><ref group=notes>Normal CD4/CD8 ratio is affected by multiple factors, including sex, age, ethnicity and genetics</ref>		A ratio of 1.5 and 2.5 is generally considered normal.<ref name="pmid29095912">{{cite journal\| author=McBride JA, Striker R\| title=Imbalance in the game of T cells: What can the CD4/CD8 T-cell ratio tell us about HIV and health? \| journal=PLoS Pathog \| year= 2017 \| volume= 13 \| issue= 11 \| pages= e1006624 \| pmid=29095912 \| doi=10.1371/journal.ppat.1006624 \| pmc=5667733 \| url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=29095912 }} </ref><ref group=notes>Normal CD4/CD8 ratio is affected by multiple factors, including sex, age, ethnicity and genetics</ref>

Mikael Häggström: Title

2023-10-18T00:06:47Z

Title

← Older revision		Revision as of 20:06, 17 October 2023
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	==		==Cell distribution==
	[[File:Flow cytometric gating by side scatter and CD45.png\|thumb\|Flow cytometry gating into main categories of blood cells by side scatter and CD45, in a case with relatively normal distributions.]]		[[File:Flow cytometric gating by side scatter and CD45.png\|thumb\|Flow cytometry gating into main categories of blood cells by side scatter and CD45, in a case with relatively normal distributions.]]
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Mikael Häggström: +Image

2023-10-18T00:05:25Z

+Image

← Older revision		Revision as of 20:05, 17 October 2023
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			[[File:Flow cytometric gating by side scatter and CD45.png\|thumb\|Flow cytometry gating into main categories of blood cells by side scatter and CD45, in a case with relatively normal distributions.]]
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	\|+ Reference ranges for leukocytes in bone marrow aspirates of healthy individuals<ref name="pmid18942105">{{cite journal\| author=Brooimans RA, Kraan J, van Putten W, Cornelissen JJ, Löwenberg B, Gratama JW\| title=Flow cytometric differential of leukocyte populations in normal bone marrow: influence of peripheral blood contamination. \| journal=Cytometry B Clin Cytom \| year= 2009 \| volume= 76 \| issue= 1 \| pages= 18-26 \| pmid=18942105 \| doi=10.1002/cyto.b.20439 \| pmc= \| url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=18942105 }} </ref>		\|+ Reference ranges for leukocytes in bone marrow aspirates of healthy individuals<ref name="pmid18942105">{{cite journal\| author=Brooimans RA, Kraan J, van Putten W, Cornelissen JJ, Löwenberg B, Gratama JW\| title=Flow cytometric differential of leukocyte populations in normal bone marrow: influence of peripheral blood contamination. \| journal=Cytometry B Clin Cytom \| year= 2009 \| volume= 76 \| issue= 1 \| pages= 18-26 \| pmid=18942105 \| doi=10.1002/cyto.b.20439 \| pmc= \| url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=18942105 }} </ref>
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	\| 0.7 \|\| 1.8 \|\| 1.8 \|\| 2.8		\| 0.7 \|\| 1.8 \|\| 1.8 \|\| 2.8
	\|-		\|-
	! Immature cells		! Hematogones/Immature cells
	\| 2.5 \|\| 5.9 \|\| 5.9 \|\| 9.2		\| 2.5 \|\| 5.9 \|\| 5.9 \|\| 9.2
	\|-		\|-
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	\| 1.4 \|\| 2.5 \|\| 2.8 \|\| 4.5		\| 1.4 \|\| 2.5 \|\| 2.8 \|\| 4.5
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	==CD4/CD8 ratio==		==CD4/CD8 ratio==
	A ratio of 1.5 and 2.5 is generally considered normal.<ref name="pmid29095912">{{cite journal\| author=McBride JA, Striker R\| title=Imbalance in the game of T cells: What can the CD4/CD8 T-cell ratio tell us about HIV and health? \| journal=PLoS Pathog \| year= 2017 \| volume= 13 \| issue= 11 \| pages= e1006624 \| pmid=29095912 \| doi=10.1371/journal.ppat.1006624 \| pmc=5667733 \| url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=29095912 }} </ref><ref group=notes>Normal CD4/CD8 ratio is affected by multiple factors, including sex, age, ethnicity and genetics</ref>		A ratio of 1.5 and 2.5 is generally considered normal.<ref name="pmid29095912">{{cite journal\| author=McBride JA, Striker R\| title=Imbalance in the game of T cells: What can the CD4/CD8 T-cell ratio tell us about HIV and health? \| journal=PLoS Pathog \| year= 2017 \| volume= 13 \| issue= 11 \| pages= e1006624 \| pmid=29095912 \| doi=10.1371/journal.ppat.1006624 \| pmc=5667733 \| url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=29095912 }} </ref><ref group=notes>Normal CD4/CD8 ratio is affected by multiple factors, including sex, age, ethnicity and genetics</ref>

Mikael Häggström:

2022-10-23T17:17:04Z

<br>

← Older revision		Revision as of 13:17, 23 October 2022
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	\|+ Reference ranges for leukocytes in bone marrow aspirates of healthy individuals<ref name="pmid18942105">{{cite journal\| author=Brooimans RA, Kraan J, van Putten W, Cornelissen JJ, Löwenberg B, Gratama JW\| title=Flow cytometric differential of leukocyte populations in normal bone marrow: influence of peripheral blood contamination. \| journal=Cytometry B Clin Cytom \| year= 2009 \| volume= 76 \| issue= 1 \| pages= 18-26 \| pmid=18942105 \| doi=10.1002/cyto.b.20439 \| pmc= \| url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=18942105 }} </ref>		\|+ Reference ranges for leukocytes in bone marrow aspirates of healthy individuals<ref name="pmid18942105">{{cite journal\| author=Brooimans RA, Kraan J, van Putten W, Cornelissen JJ, Löwenberg B, Gratama JW\| title=Flow cytometric differential of leukocyte populations in normal bone marrow: influence of peripheral blood contamination. \| journal=Cytometry B Clin Cytom \| year= 2009 \| volume= 76 \| issue= 1 \| pages= 18-26 \| pmid=18942105 \| doi=10.1002/cyto.b.20439 \| pmc= \| url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=18942105 }} </ref>
	\|-		\|-
	! Cell type !! 5th percentile !! Median !! Mean !! 95th percentile		! Cell type !! 5th<br>percentile !! Median !! Mean !! 95th<br>percentile
	\|-		\|-
	! CD34+ cells		! CD34+ cells

Mikael Häggström: +Reference ranges

2022-10-23T17:16:27Z

+Reference ranges

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 ==CD4/CD8 ratio==
 A ratio of 1.5 and 2.5 is generally considered normal.<ref name="pmid29095912">{{cite journal| author=McBride JA, Striker R| title=Imbalance in the game of T cells: What can the CD4/CD8 T-cell ratio tell us about HIV and health? | journal=PLoS Pathog | year= 2017 | volume= 13 | issue= 11 | pages= e1006624 | pmid=29095912 | doi=10.1371/journal.ppat.1006624 | pmc=5667733 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=29095912  }} </ref><ref group=notes>Normal CD4/CD8 ratio is affected by multiple factors, including sex, age, ethnicity and genetics</ref>

Mikael Häggström: +Kappa/lambda

2022-01-05T00:03:33Z

+Kappa/lambda

← Older revision		Revision as of 20:03, 4 January 2022
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	==CD4/CD8 ratio==		==CD4/CD8 ratio==
	A ratio of 1.5 and 2.5 is generally considered normal.<ref name="pmid29095912">{{cite journal\| author=McBride JA, Striker R\| title=Imbalance in the game of T cells: What can the CD4/CD8 T-cell ratio tell us about HIV and health? \| journal=PLoS Pathog \| year= 2017 \| volume= 13 \| issue= 11 \| pages= e1006624 \| pmid=29095912 \| doi=10.1371/journal.ppat.1006624 \| pmc=5667733 \| url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=29095912 }} </ref><ref group=notes>Normal CD4/CD8 ratio is affected by multiple factors, including sex, age, ethnicity and genetics</ref>		A ratio of 1.5 and 2.5 is generally considered normal.<ref name="pmid29095912">{{cite journal\| author=McBride JA, Striker R\| title=Imbalance in the game of T cells: What can the CD4/CD8 T-cell ratio tell us about HIV and health? \| journal=PLoS Pathog \| year= 2017 \| volume= 13 \| issue= 11 \| pages= e1006624 \| pmid=29095912 \| doi=10.1371/journal.ppat.1006624 \| pmc=5667733 \| url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=29095912 }} </ref><ref group=notes>Normal CD4/CD8 ratio is affected by multiple factors, including sex, age, ethnicity and genetics</ref>

			==Kappa/lambda ratio==
			Normal range: 1.35 - 2.95.<ref name="pmid31949873">{{cite journal\| author=Jiang AS, Wu Z, Wei EX, Ni H, You B, Yang T \| display-authors=etal\| title=Plasma cell myeloma with dual expression of kappa and lambda light chains. \| journal=Int J Clin Exp Pathol \| year= 2018 \| volume= 11 \| issue= 9 \| pages= 4718-4723 \| pmid=31949873 \| doi= \| pmc=6962953 \| url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=31949873 }} </ref>
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Mikael Häggström: Started

2022-01-04T17:56:47Z

Started

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==CD4/CD8 ratio==
A ratio of 1.5 and 2.5 is generally considered normal.<ref name="pmid29095912">{{cite journal| author=McBride JA, Striker R| title=Imbalance in the game of T cells: What can the CD4/CD8 T-cell ratio tell us about HIV and health? | journal=PLoS Pathog | year= 2017 | volume= 13 | issue= 11 | pages= e1006624 | pmid=29095912 | doi=10.1371/journal.ppat.1006624 | pmc=5667733 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=29095912 }} </ref><ref group=notes>Normal CD4/CD8 ratio is affected by multiple factors, including sex, age, ethnicity and genetics</ref>
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