Lymphoma - Revision history

Mikael Häggström: /* Gross processing */ Detail

2023-07-13T22:53:25Z

Gross processing: Detail

← Older revision		Revision as of 18:53, 13 July 2023
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	Whenever there is a suspicion of lymphoma, perform the following before putting the specimen in formalin:		Whenever there is a suspicion of lymphoma, perform the following before putting the specimen in formalin:
	*Take a small sample for '''flow cytometry'''.		*Take a small sample for '''flow cytometry'''.
	*Make a "'''touch prep'''": lightly press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately (to avoid drying artifacts) and stain with H&E.		*Make a "'''touch prep'''": lightly press a glass slide against the cut surface of the lymph node, lift the glass slide, apply cytologic fixative solution immediately (to avoid drying artifacts) and stain with H&E.
	*Submit sections for histologic processing with '''H&E staining'''.		*Submit sections for histologic processing with '''H&E staining'''.

Mikael Häggström: /* Presentations */ Tonsil

2022-10-28T19:54:59Z

Presentations: Tonsil

← Older revision		Revision as of 15:54, 28 October 2022
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	*[[Lymph node]]		*[[Lymph node]]
	*[[Tumor]]		*[[Tumor]]
			*[[Tonsil]]

	==Gross processing==		==Gross processing==

Mikael Häggström: /* Gross processing */ Bolded

2022-10-28T19:54:37Z

Gross processing: Bolded

← Older revision		Revision as of 15:54, 28 October 2022
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	[[File:Touch prep on a lymph node.jpg\|thumb\|220px\|Making a "touch prep".]]		[[File:Touch prep on a lymph node.jpg\|thumb\|220px\|Making a "touch prep".]]
	Whenever there is a suspicion of lymphoma, perform the following before putting the specimen in formalin:		Whenever there is a suspicion of lymphoma, perform the following before putting the specimen in formalin:
	*Take a small sample for flow cytometry.		*Take a small sample for '''flow cytometry'''.
	*Make a "touch prep": lightly press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately (to avoid drying artifacts) and stain with H&E.		*Make a "'''touch prep'''": lightly press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately (to avoid drying artifacts) and stain with H&E.
	*Submit sections for histologic processing ~~and~~ H&E staining.		*Submit sections for histologic processing with '''H&E staining'''.

	Otherwise, as per location or specimen type, such as '''[[lymph node]]''' or '''[[tumor]]'''.		Otherwise, as per location or specimen type, such as '''[[lymph node]]''' or '''[[tumor]]'''.

Mikael Häggström: /* Gross processing */ +Image

2022-09-01T21:26:16Z

Gross processing: +Image

← Older revision		Revision as of 17:26, 1 September 2022
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	==Gross processing==		==Gross processing==
			[[File:Touch prep on a lymph node.jpg\|thumb\|220px\|Making a "touch prep".]]
	Whenever there is a suspicion of lymphoma, perform the following before putting the specimen in formalin:		Whenever there is a suspicion of lymphoma, perform the following before putting the specimen in formalin:
	*Take a small sample for flow cytometry.		*Take a small sample for flow cytometry.
	*Make a touch prep: press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately and stain with H&E.		*Make a "touch prep": lightly press a glass slide against the cut surface of the lymph node, apply cytologic fixative solution immediately (to avoid drying artifacts) and stain with H&E.
	*Submit sections for histologic processing and H&E staining.		*Submit sections for histologic processing and H&E staining.

Mikael Häggström: /* Microscopic examination */ +cd23

2022-05-18T12:13:06Z

Microscopic examination: +cd23

← Older revision		Revision as of 08:13, 18 May 2022
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	*{{Moderate-begin}}'''CD5''', mainly to suggest chronic lymphocytic leukemia or mantle zone lymphoma if it positive in the CD20 positive cells.{{Moderate-end}}		*{{Moderate-begin}}'''CD5''', mainly to suggest chronic lymphocytic leukemia or mantle zone lymphoma if it positive in the CD20 positive cells.{{Moderate-end}}
	*{{Moderate-begin}}'''CD10''', essentially confirming a [[follicular lymphoma]] if stains the BCL2 positive cells of secondary follicles.{{Moderate-end}}		*{{Moderate-begin}}'''CD10''', essentially confirming a [[follicular lymphoma]] if stains the BCL2 positive cells of secondary follicles.{{Moderate-end}}
			*{{Moderate-begin}}'''CD23''', suggesting a small lymphocytic lymphoma (SLL) / chronic lymphocytic leukemia (CLL) if it is present in CD20 and CD5 positive cells.{{Moderate-end}}
	*{{Moderate-begin}}'''Cyclin D''', suggesting a [[mantle cell lymphoma]] if it is present in CD20 and CD5 positive cells.{{Moderate-end}}		*{{Moderate-begin}}'''Cyclin D''', suggesting a [[mantle cell lymphoma]] if it is present in CD20 and CD5 positive cells.{{Moderate-end}}

Mikael Häggström: /* Small to medium sized lymphocytes */ Integrated

2022-01-22T16:19:10Z

Small to medium sized lymphocytes: Integrated

← Older revision		Revision as of 12:19, 22 January 2022
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	*{{Moderate-begin}}'''Cyclin D''', suggesting a [[mantle cell lymphoma]] if it is present in CD20 and CD5 positive cells.{{Moderate-end}}		*{{Moderate-begin}}'''Cyclin D''', suggesting a [[mantle cell lymphoma]] if it is present in CD20 and CD5 positive cells.{{Moderate-end}}

	~~Overview of main B cell lymphomas (~~CD20 positive cells predominant):		If CD20 positive cells (B cells) are predominant, attempt to fit their pattern into one of the following:<ref name="Attanoos2018">{{cite book\|last1=Attanoos\|first1=Richard\|title=Practical Pathology of Serous Membranes\|chapter=Lymphoid Malignancies of the Pleura and Peritoneum\|year=2018\|pages=203–208\|doi=10.1017/9781316402009.016\|isbn=9781316402009}}</ref>
	{\|class="wikitable" style="text-align: center"		{\|class="wikitable" style="text-align: center"
	\|+ Main [[immunohistochemistry]] markers in common types of B-cell lymphoma.<ref name="Attanoos2018">{{cite book\|last1=Attanoos\|first1=Richard\|title=Practical Pathology of Serous Membranes\|chapter=Lymphoid Malignancies of the Pleura and Peritoneum\|year=2018\|pages=203–208\|doi=10.1017/9781316402009.016\|isbn=9781316402009}}</ref>
	!		!
	! [[Follicular lymphoma]]		! [[Follicular lymphoma]]

Mikael Häggström: /* Microscopic examination */ Specified

2022-01-22T16:17:57Z

Microscopic examination: Specified

← Older revision		Revision as of 12:17, 22 January 2022
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	==Microscopic examination==		==Microscopic examination==
	On the histologically processed slides, first compare the lymphoid cells with endothelial cells, and designate the lymphoid cells as '''large''' if ~~they~~ are larger than the endothelial cells, otherwise as small. In addition, you generally need to order immunohistochemistry, consisting of:		On the histologically processed slides, first compare the lymphoid cells with endothelial cells, and designate the lymphoid cells as '''large''' if a significant amount are larger than the transverse diameter of endothelial cells, otherwise as medium to small.

			===Small to medium sized lymphocytes===
			In addition, you generally need to order immunohistochemistry, consisting of:
	*'''CD3''', to raise the possibility of a T cell lymphoma if it stains a vast majority of cells.		*'''CD3''', to raise the possibility of a T cell lymphoma if it stains a vast majority of cells.
	*'''CD20''', to raise the possibility of a B cell lymphoma if predominant.		*'''CD20''', to raise the possibility of a B cell lymphoma if predominant.

Mikael Häggström: /* Microscopic examination */ main

2022-01-10T00:04:34Z

Microscopic examination: main

← Older revision		Revision as of 20:04, 9 January 2022
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	*{{Moderate-begin}}'''Cyclin D''', suggesting a [[mantle cell lymphoma]] if it is present in CD20 and CD5 positive cells.{{Moderate-end}}		*{{Moderate-begin}}'''Cyclin D''', suggesting a [[mantle cell lymphoma]] if it is present in CD20 and CD5 positive cells.{{Moderate-end}}

	Overview of B cell lymphomas (CD20 positive cells predominant):		Overview of main B cell lymphomas (CD20 positive cells predominant):
	{\|class="wikitable" style="text-align: center"		{\|class="wikitable" style="text-align: center"
	\|+ Main [[immunohistochemistry]] markers in common types of B-cell lymphoma.<ref name="Attanoos2018">{{cite book\|last1=Attanoos\|first1=Richard\|title=Practical Pathology of Serous Membranes\|chapter=Lymphoid Malignancies of the Pleura and Peritoneum\|year=2018\|pages=203–208\|doi=10.1017/9781316402009.016\|isbn=9781316402009}}</ref>		\|+ Main [[immunohistochemistry]] markers in common types of B-cell lymphoma.<ref name="Attanoos2018">{{cite book\|last1=Attanoos\|first1=Richard\|title=Practical Pathology of Serous Membranes\|chapter=Lymphoid Malignancies of the Pleura and Peritoneum\|year=2018\|pages=203–208\|doi=10.1017/9781316402009.016\|isbn=9781316402009}}</ref>

Mikael Häggström: /* Microscopic examination */ Amended

2022-01-10T00:04:10Z

Microscopic examination: Amended

← Older revision		Revision as of 20:04, 9 January 2022
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	!		!
	! [[Follicular lymphoma]]		! [[Follicular lymphoma]]
	! [[Marginal zone B-cell lymphoma]] (MZL) or ~~[[mucosa-associated lymphatic tissue lymphoma\|~~mucosa-associated lymphatic tissue (MALT) lymphoma]]		! Marginal zone B-cell lymphoma (MZL) or mucosa-associated lymphatic tissue (MALT) lymphoma
	! [[Small lymphocytic lymphoma]] (SLL) / [[chronic lymphocytic leukemia]] (CLL)		! Small lymphocytic lymphoma (SLL) / chronic lymphocytic leukemia (CLL)
	! [[Mantle cell lymphoma]] (MCL)		! Mantle cell lymphoma (MCL)
	\|-		\|-
	~~\| [[~~CD5 ~~(protein)\|CD5]]~~		! CD5
	\|style="background: lightpink"\| -		\|style="background: lightpink"\| -
	\|style="background: lightpink"\| -		\|style="background: lightpink"\| -
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	\|style="background: lightgreen"\| +		\|style="background: lightgreen"\| +
	\|-		\|-
	~~\| [[~~CD10]]		! CD10
	\|style="background: lightgreen"\| +		\|style="background: lightgreen"\| +
	\|style="background: lightpink"\| -		\|style="background: lightpink"\| -
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	\|style="background: lightpink"\| -		\|style="background: lightpink"\| -
	\|-		\|-
	~~\| [[~~CD23]]		! CD23
	\|style="background: lightpink"\| -		\|style="background: lightpink"\| -
	\|style="background: lightpink"\| -		\|style="background: lightpink"\| -
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	\|style="background: lightpink"\| -		\|style="background: lightpink"\| -
	\|-		\|-
	~~\| [[~~Cyclin D1]]		! Cyclin D1
	\|style="background: lightpink"\| -		\|style="background: lightpink"\| -
	\|style="background: lightpink"\| -		\|style="background: lightpink"\| -

Mikael Häggström: /* Microscopic examination */ expanded

2022-01-10T00:03:13Z

Microscopic examination: expanded

← Older revision		Revision as of 20:03, 9 January 2022
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	*{{Moderate-begin}}'''CD5''', mainly to suggest chronic lymphocytic leukemia or mantle zone lymphoma if it positive in the CD20 positive cells.{{Moderate-end}}		*{{Moderate-begin}}'''CD5''', mainly to suggest chronic lymphocytic leukemia or mantle zone lymphoma if it positive in the CD20 positive cells.{{Moderate-end}}
	*{{Moderate-begin}}'''CD10''', essentially confirming a [[follicular lymphoma]] if stains the BCL2 positive cells of secondary follicles.{{Moderate-end}}		*{{Moderate-begin}}'''CD10''', essentially confirming a [[follicular lymphoma]] if stains the BCL2 positive cells of secondary follicles.{{Moderate-end}}
	*{{Moderate-begin}}'''Cyclin D''', suggesting a [[mantle cell lymphoma]] if it is present in CD20 and CD5 positive cells.		*{{Moderate-begin}}'''Cyclin D''', suggesting a [[mantle cell lymphoma]] if it is present in CD20 and CD5 positive cells.{{Moderate-end}}

	Overview of B cell lymphomas (CD20 positive cells predominant):		Overview of B cell lymphomas (CD20 positive cells predominant):