Microbiology - Revision history

Mikael Häggström: /* Learning microbiology */ Template

2024-03-15T20:33:08Z

Learning microbiology: Template

← Older revision		Revision as of 16:33, 15 March 2024
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	==Learning microbiology==		==Learning microbiology==
	~~For clinical pathology trainees,~~ microbiology is a subspecialty with a particularly high discrepancy between everyday practice and what is tested for on exams. In particular, exams have a tendency to ask to identify uncommon bacteria based on morphologic and biochemical characteristics, whereas in everyday practice there are only a limited number of bacteria that are identified by such testing, whereas the rest are generally identified by multitarget assays and/or MALDI-TOF. Thus, a lot of effort should be put on identifying what is actually memorization-worthy before starting to memorize microbiology-related content. {{further\|Learning pathology}}		{{Learning microbiology}}
			{{further\|Learning pathology}}

	==Example algorithm==		==Example algorithm==

Mikael Häggström: particularly

2023-07-27T17:48:53Z

particularly

← Older revision		Revision as of 13:48, 27 July 2023
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	[[File:Early Proteus-looking colonies.jpg\|thumb\|230px\|Early Proteus-looking colonies]]		[[File:Early Proteus-looking colonies.jpg\|thumb\|230px\|Early Proteus-looking colonies]]
	The microbiology technician asks you to have a look at a recent wound culture in a patient whose previous blood culture showed gram positive bacteria. The blood agar (shown) grows early colonies of what mainly looks like Proteus (which is gram negative). Is it acceptable to take a sample for MALDI-TOF or multitargeted assay without further bench testing?		The microbiology technician asks you to have a look at a recent wound culture in a patient whose previous blood culture showed gram positive bacteria. The blood agar (shown) grows early colonies of what mainly looks like Proteus (which is gram negative). Is it acceptable to take a sample for MALDI-TOF or multitargeted assay without further bench testing?
	;'''Answer''': No. We want to rule out gram positives in the culture, but Proteus tends to swarm all over blood agars and get mixed with other cultures, whereas the mentioned machines should receive pure cultures. In this case, one or more samples should be plated on an agar that inhibits gram negatives (such as CNA) in order to detect gram positives.		;'''Answer''': No. In this case, we want to particularly rule out gram positives in the culture, but Proteus tends to swarm all over blood agars and get mixed with other cultures, whereas the mentioned machines should receive pure cultures. In this case, one or more samples should be plated on an agar that inhibits gram negatives (such as CNA) in order to detect gram positives.
	{{Question-end}}		{{Question-end}}
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Mikael Häggström: Q

2023-07-27T17:38:00Z

← Older revision		Revision as of 13:38, 27 July 2023
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	Following is an example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. The grey box near top left shows a Venn diagram of what culture media are routinely used for various sources or purposes. The included pathways are the most common ones, but are not mandatory, as there are multiple exceptions to them.		Following is an example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. The grey box near top left shows a Venn diagram of what culture media are routinely used for various sources or purposes. The included pathways are the most common ones, but are not mandatory, as there are multiple exceptions to them.
	[[File:Diagnostic algorithm of possible bacterial infection.png\|thumb\|center\|900px]]		[[File:Diagnostic algorithm of possible bacterial infection.png\|thumb\|center\|900px]]
			{{Question\|title=Proteus-looking culture}}
			[[File:Early Proteus-looking colonies.jpg\|thumb\|230px\|Early Proteus-looking colonies]]
			The microbiology technician asks you to have a look at a recent wound culture in a patient whose previous blood culture showed gram positive bacteria. The blood agar (shown) grows early colonies of what mainly looks like Proteus (which is gram negative). Is it acceptable to take a sample for MALDI-TOF or multitargeted assay without further bench testing?
			;'''Answer''': No. We want to rule out gram positives in the culture, but Proteus tends to swarm all over blood agars and get mixed with other cultures, whereas the mentioned machines should receive pure cultures. In this case, one or more samples should be plated on an agar that inhibits gram negatives (such as CNA) in order to detect gram positives.
			{{Question-end}}
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Mikael Häggström: /* Example algorithm */ Size

2023-07-27T17:14:31Z

Example algorithm: Size

← Older revision		Revision as of 13:14, 27 July 2023
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	==Example algorithm==		==Example algorithm==
	Following is an example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. The grey box near top left shows a Venn diagram of what culture media are routinely used for various sources or purposes. The included pathways are the most common ones, but are not mandatory, as there are multiple exceptions to them.		Following is an example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. The grey box near top left shows a Venn diagram of what culture media are routinely used for various sources or purposes. The included pathways are the most common ones, but are not mandatory, as there are multiple exceptions to them.
	[[File:Diagnostic algorithm of possible bacterial infection.png\|thumb\|center\|~~800px~~]]		[[File:Diagnostic algorithm of possible bacterial infection.png\|thumb\|center\|900px]]
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Mikael Häggström: /* Example algorithm */ Size

2023-07-27T17:14:09Z

Example algorithm: Size

← Older revision		Revision as of 13:14, 27 July 2023
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	==Example algorithm==		==Example algorithm==
	Following is an example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. The grey box near top left shows a Venn diagram of what culture media are routinely used for various sources or purposes. The included pathways are the most common ones, but are not mandatory, as there are multiple exceptions to them.		Following is an example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. The grey box near top left shows a Venn diagram of what culture media are routinely used for various sources or purposes. The included pathways are the most common ones, but are not mandatory, as there are multiple exceptions to them.
	[[File:Diagnostic algorithm of possible bacterial infection.png\|thumb\|center\|~~1000px~~]]		[[File:Diagnostic algorithm of possible bacterial infection.png\|thumb\|center\|800px]]
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Mikael Häggström: Started

2023-07-27T16:51:29Z

Started

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==Learning microbiology==
For clinical pathology trainees, microbiology is a subspecialty with a particularly high discrepancy between everyday practice and what is tested for on exams. In particular, exams have a tendency to ask to identify uncommon bacteria based on morphologic and biochemical characteristics, whereas in everyday practice there are only a limited number of bacteria that are identified by such testing, whereas the rest are generally identified by multitarget assays and/or MALDI-TOF. Thus, a lot of effort should be put on identifying what is actually memorization-worthy before starting to memorize microbiology-related content. {{further|Learning pathology}}

==Example algorithm==
Following is an example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. The grey box near top left shows a Venn diagram of what culture media are routinely used for various sources or purposes. The included pathways are the most common ones, but are not mandatory, as there are multiple exceptions to them.
[[File:Diagnostic algorithm of possible bacterial infection.png|thumb|center|1000px]]
{{Bottom}}