Flow cytometry: Difference between revisions
Jump to navigation
Jump to search
Started |
+Viability |
||
| (5 intermediate revisions by the same user not shown) | |||
| Line 3: | Line 3: | ||
|author2= | |author2= | ||
}} | }} | ||
==Cell distribution== | |||
[[File:Flow cytometric gating by side scatter and CD45.png|thumb|Flow cytometry gating into main categories of blood cells by side scatter and CD45, in a case with relatively normal distributions.]] | |||
{| class=wikitable align=left | |||
|+ Reference ranges for leukocytes in bone marrow aspirates of healthy individuals<ref name="pmid18942105">{{cite journal| author=Brooimans RA, Kraan J, van Putten W, Cornelissen JJ, Löwenberg B, Gratama JW| title=Flow cytometric differential of leukocyte populations in normal bone marrow: influence of peripheral blood contamination. | journal=Cytometry B Clin Cytom | year= 2009 | volume= 76 | issue= 1 | pages= 18-26 | pmid=18942105 | doi=10.1002/cyto.b.20439 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=18942105 }} </ref> | |||
|- | |||
! Cell type !! 5th<br>percentile !! Median !! Mean !! 95th<br>percentile | |||
|- | |||
! CD34+ cells | |||
| 0.7 || 1.8 || 1.8 || 2.8 | |||
|- | |||
! Hematogones/Immature cells | |||
| 2.5 || 5.9 || 5.9 || 9.2 | |||
|- | |||
! Lymphocytes | |||
| 5.7 || 13.4 || 14.2 || 25 | |||
|- | |||
! Granulocytes | |||
| 59.5 || 73.7 || 73.4 || 85.5 | |||
|- | |||
! Monocytes | |||
| 2 || 3.5 || 3.7 || 5.8 | |||
|- | |||
! Remaining cells | |||
| 1.4 || 2.5 || 2.8 || 4.5 | |||
|} | |||
<br clear=all> | |||
==Viability== | |||
[[File:Flow cytometric viability by 7-AAD.png|thumb|Flow cytometry using 7-Aminoactinomycin D (7-AAD), wherein a lower signal indicates viable cells. Therefore, this case shows good viability.]] | |||
Viability of the cells in flow cytometry should be around 95% but not less than 90%.<ref>{{cite web|url=https://medicine.yale.edu/immuno/flowcore/protocols/analysis/|title=Flow cytometry (FACS) staining protocol (Cell surface staining)|website=Yale School of Medicine - Yale Flow Cytometry|accessdate=2023-10-17}}</ref> | |||
==CD4/CD8 ratio== | ==CD4/CD8 ratio== | ||
A ratio of 1.5 and 2.5 is generally considered normal.<ref name="pmid29095912">{{cite journal| author=McBride JA, Striker R| title=Imbalance in the game of T cells: What can the CD4/CD8 T-cell ratio tell us about HIV and health? | journal=PLoS Pathog | year= 2017 | volume= 13 | issue= 11 | pages= e1006624 | pmid=29095912 | doi=10.1371/journal.ppat.1006624 | pmc=5667733 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=29095912 }} </ref><ref group=notes>Normal CD4/CD8 ratio is affected by multiple factors, including sex, age, ethnicity and genetics</ref> | A ratio of 1.5 and 2.5 is generally considered normal.<ref name="pmid29095912">{{cite journal| author=McBride JA, Striker R| title=Imbalance in the game of T cells: What can the CD4/CD8 T-cell ratio tell us about HIV and health? | journal=PLoS Pathog | year= 2017 | volume= 13 | issue= 11 | pages= e1006624 | pmid=29095912 | doi=10.1371/journal.ppat.1006624 | pmc=5667733 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=29095912 }} </ref><ref group=notes>Normal CD4/CD8 ratio is affected by multiple factors, including sex, age, ethnicity and genetics</ref> | ||
==Kappa/lambda ratio== | |||
Normal range: 1.35 - 2.95.<ref name="pmid31949873">{{cite journal| author=Jiang AS, Wu Z, Wei EX, Ni H, You B, Yang T | display-authors=etal| title=Plasma cell myeloma with dual expression of kappa and lambda light chains. | journal=Int J Clin Exp Pathol | year= 2018 | volume= 11 | issue= 9 | pages= 4718-4723 | pmid=31949873 | doi= | pmc=6962953 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=31949873 }} </ref> | |||
{{Bottom}} | {{Bottom}} | ||
Latest revision as of 20:48, 17 October 2023
Author:
Mikael Häggström, M.D. [note 1]
Cell distribution
| Cell type | 5th percentile |
Median | Mean | 95th percentile |
|---|---|---|---|---|
| CD34+ cells | 0.7 | 1.8 | 1.8 | 2.8 |
| Hematogones/Immature cells | 2.5 | 5.9 | 5.9 | 9.2 |
| Lymphocytes | 5.7 | 13.4 | 14.2 | 25 |
| Granulocytes | 59.5 | 73.7 | 73.4 | 85.5 |
| Monocytes | 2 | 3.5 | 3.7 | 5.8 |
| Remaining cells | 1.4 | 2.5 | 2.8 | 4.5 |
Viability
Viability of the cells in flow cytometry should be around 95% but not less than 90%.[2]
CD4/CD8 ratio
A ratio of 1.5 and 2.5 is generally considered normal.[3][notes 1]
Kappa/lambda ratio
Normal range: 1.35 - 2.95.[4]
Notes
- ↑ Normal CD4/CD8 ratio is affected by multiple factors, including sex, age, ethnicity and genetics
- ↑ For a full list of contributors, see article history. Creators of images are attributed at the image description pages, seen by clicking on the images. See Patholines:Authorship for details.
Main page
References
- ↑ Brooimans RA, Kraan J, van Putten W, Cornelissen JJ, Löwenberg B, Gratama JW (2009). "Flow cytometric differential of leukocyte populations in normal bone marrow: influence of peripheral blood contamination. ". Cytometry B Clin Cytom 76 (1): 18-26. doi:. PMID 18942105. Archived from the original. .
- ↑ . Flow cytometry (FACS) staining protocol (Cell surface staining). Yale School of Medicine - Yale Flow Cytometry. Retrieved on 2023-10-17.
- ↑ McBride JA, Striker R (2017). "Imbalance in the game of T cells: What can the CD4/CD8 T-cell ratio tell us about HIV and health? ". PLoS Pathog 13 (11): e1006624. doi:. PMID 29095912. PMC: 5667733. Archived from the original. .
- ↑ Jiang AS, Wu Z, Wei EX, Ni H, You B, Yang T (2018). "Plasma cell myeloma with dual expression of kappa and lambda light chains. ". Int J Clin Exp Pathol 11 (9): 4718-4723. PMID 31949873. PMC: 6962953. Archived from the original. .
Image sources